FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs7_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs7_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9009654
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT178220.19781003798814029TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32050.033.4268211
ACACGTC32950.032.6471413
ACGTCTG33500.031.91414515
CACACGT34150.031.50012612
CACGTCT34750.030.70284814
GATCGGA34550.030.325881
GAACTCC35450.029.72442221
CGTCTGA36150.029.4529516
AGCACAC36600.029.33124410
GAGCACA38150.028.4278559
CAGTCAC37550.028.17908327
TCCAGTC37750.028.02978925
CACAGTC38200.027.3540731
GTCACAG38750.027.30659329
CTCGTAT34100.027.15950644
CCAGTCA38800.027.15785826
AGAGCAC40600.026.7128288
AGTCACA40250.026.28880928
TCTCGTA35750.025.7829243
ACTCCAG42400.025.05981323
Produced by FastQC (version 0.11.2)