FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs6_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs6_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980891
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2383232.9861703411310843TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC167130.20941270843067525TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC103420.1295845288452129TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG341250.042.7274911
CGTCTGA337800.042.7142516
ACACGTC340600.042.6733913
ACGTCTG339650.042.6305615
CACACGT341000.042.61688612
CACGTCT339900.042.53448514
CGTATGC329250.042.36075644
CAGTCAC340100.042.04428527
TCCAGTC341250.041.97350725
CCAGTCA340950.041.95882426
AGCACAC347300.041.95150410
GAACTCC344050.041.8807621
AGTCACC341300.041.81266428
GAGCACA349550.041.7569859
TCTCGTA286950.041.65179441
GTCTGAA346700.041.64947517
GTCACCT340600.041.63378529
ATCTCGT284500.041.58519740
TCGTATG333200.041.5680843
CTCGTAT294050.041.5513342
Produced by FastQC (version 0.11.2)