FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs5_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs5_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7576246
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG19850.023.05119115
ACACGTC19950.023.04591613
CACGTCT20500.022.32030114
CGTCTGA20700.022.10464516
TCCAGTC20300.021.89015225
GAACTCC20750.021.83934821
CATCTCG14750.021.7746639
CAGTCAC21850.020.13594427
CCAGTCA22500.019.7497826
AGCACAC23400.019.55410810
CACACGT23600.019.38839712
CCAGATC21250.019.25504733
GTCTGAA25200.017.89550217
GATCGGA25600.017.2832241
CTCGTAT19350.017.28037542
AGTCACC24950.017.19323228
GAGCACA27200.017.145799
TGAACTC28150.016.09827620
AGAGCAC29100.016.0264118
TCGGAAG28850.015.8682433
Produced by FastQC (version 0.11.2)