FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs4_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs4_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7222055
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC112890.15631285001291184TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC18350.033.44725829
TCACGCC19800.030.33121930
CACGCCA20650.029.40230831
AGTCACG21850.028.4922928
GCACACG23100.027.23619811
CACACGT22950.027.12665212
ACGTCTG23600.026.4727315
CACGTCT23650.026.32374614
ACACGTC23900.025.95634813
TATCTCG22050.025.93941539
GATCGGA23050.025.6883051
CGTATGC23450.025.61015544
CAGTCAC24500.025.41047527
CGTCTGA24400.024.97367116
TCCAGTC25250.024.4814625
CCAGTCA26150.023.8912626
GAACTCC26700.023.15194321
AGCACAC27250.022.68465810
CTCGTAT25700.022.51204942
GAGCACA28100.022.1550399
Produced by FastQC (version 0.11.2)