FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs3_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs3_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6615353
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10300.017.29990211
GATCGGA11450.015.7638971
ACGTCTG12050.014.97002815
CGTCTGA12200.014.96628816
GTCACAC12550.014.72429929
CACACGT12800.014.26474312
CACGTCT13000.013.87606514
TCCAGTC14700.013.31900425
ACACGTC13750.013.27917913
CACACAG13550.013.15047931
CGTATGC13200.012.99919644
CAGTCAC14700.012.72039727
AGCACAC14900.012.69720210
AGTCACA16750.011.82025428
CACAGTG15300.011.7901233
GAACTCC15800.011.69539121
GAGCACA17600.011.6242819
CCAGTCA16200.011.54258326
AGAGCAC17550.011.1560058
GATCTCG12700.010.91279339
Produced by FastQC (version 0.11.2)