FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs2_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs2_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143678
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12800.020.27980211
ACGTCTG13300.020.01360915
ACACGTC13400.019.53591713
CACGTCT13750.019.1986314
ACTGACC13300.018.85579732
CGTCTGA14700.018.25719816
GATCGGA13300.018.2059141
TCCAGTC14800.017.53928825
CAGTCAC15150.017.13408927
CACACGT15600.016.9218712
CGTATGC15300.015.95953444
CCAGTCA16550.015.95052226
GAACTCC17200.015.21984321
AGCACAC17250.015.04820210
GAGCACA18150.014.6656199
AGAGCAC19050.014.3192878
CTGACCA17700.014.29276533
CACTGAC17400.014.28633631
TCGTATG18100.013.61219243
CTCGTAT15500.013.34088942
Produced by FastQC (version 0.11.2)