FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs21_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs21_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6422550
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12850.028.58970611
AGTCACG13400.027.25207928
ACACGTC14100.025.89913613
CACGTCT14150.025.49648714
GTCACGT14800.024.67417729
CACACGT14950.024.5737612
ACGTCTG15100.024.18377515
GATCGGA15650.023.0649431
CGTCTGA16450.022.86773516
CGTTTCG15900.022.55191433
GTTTCGG16550.021.7991134
AGCACAC16800.021.73677610
CAGTCAC18100.020.9048127
TCCAGTC18500.020.333925
GAACTCC18250.020.13012721
GAGCACA18550.020.041919
CCAGTCA19200.019.36340726
AGAGCAC20050.019.0912598
ACTCCAG20300.018.09742423
CTCGTAT16500.017.46547144
Produced by FastQC (version 0.11.2)