FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs20_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs20_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9268916
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT328000.35387093809027936TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG50050.036.8770328
ACGTGGC49250.036.71630532
GCACACG52850.035.5056511
GTCACGT52000.035.45183629
CACGTGG51150.035.4384731
ACGTCTG53550.035.41124315
TCACGTG51950.035.23169330
CACGTCT53650.035.18122514
ACACGTC54250.034.91377313
CACACGT54350.034.6471612
CGTCTGA55150.034.22434616
GGCCTTA52200.033.62991736
TATCTCG47000.033.1387341
CAGTCAC57000.032.76674327
TCCAGTC58250.031.9880625
CGTGGCC57650.031.3664933
CCAGTCA59300.031.31036826
GTGGCCT57650.031.13753734
CTCGTAT53700.031.01144444
AGCACAC61200.030.9848410
Produced by FastQC (version 0.11.2)