FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs1_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs1_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7106533
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC88230.12415336704972733TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18050.027.78808611
CGTCTGA18800.027.4982416
ACACGTC18750.026.98551213
ACGTCTG18950.026.46796415
CACGTCT19900.025.20459714
CACACGT20000.025.07892412
GATCGGA20250.024.783611
CCGATGT21500.023.43089533
CAGTCAC21550.023.3768627
CGTATGC22150.022.44535844
TCCAGTC22450.022.34171125
TATCTCG21050.022.05084439
AGCACAC23400.021.99890310
GAACTCC22950.021.95066521
CGATGTA23600.021.15951534
GTCACCG24150.021.04213129
ACCGATG24000.020.99017732
CACCGAT24600.020.8359231
GAGCACA24500.020.7418239
CTCGTAT22200.020.71024142
Produced by FastQC (version 0.11.2)