FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs19_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs19_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6711641
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC92320.13755205321619557TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT14900.033.0717231
GCACACG16100.031.9731211
GTCACGG15450.031.75225829
TCACGGC16100.030.4701130
AGTCACG16350.030.2735328
ACGTCTG17300.029.11953515
CGGCTAC16950.028.9421133
ACACGTC18350.028.17259213
GATCGGA17650.027.9338871
ACGGCTA17600.027.87322632
CACACGT19200.027.26909812
CGTCTGA18850.026.84179116
CGTATGC18850.026.49168244
CACGTCT19200.026.46706614
AGCACAC21000.024.93174710
CATCTCG16750.024.69096639
TCCAGTC21050.023.61862425
CAGTCAC21600.023.32276327
TCGTATG21800.023.0077143
GAACTCC22150.022.94210621
Produced by FastQC (version 0.11.2)