FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs18_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs18_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7486610
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC98150.13110072516132135TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19750.029.29460511
ACACGTC20500.028.65190713
GATCGGA20200.027.240251
ACGTCTG21100.027.10734715
CACGTCT21600.026.785414
CGTCTGA21450.026.4599216
CACACGT22300.025.84595312
CGTATGC21400.025.69936244
AGCACAC24550.023.7461610
CACTAGC24450.023.57351731
GAACTCC25050.023.36015921
CAGTCAC25000.023.23104527
TATCTCG22550.022.14498739
TCCAGTC26500.021.99909625
ACTAGCT25550.021.95589632
TCGGAAG27400.021.8463953
GAGCACA27150.021.7152089
TCGTATG24350.021.4114243
TCACTAG27100.021.34924730
CTCGTAT23550.021.20464742
Produced by FastQC (version 0.11.2)