FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs17_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs17_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8311917
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC275820.3318368073213436TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG43950.036.08881428
GTCACGA43800.036.0617329
GCACACG44750.035.6896611
CGATCAG45450.034.70436533
ACACGTC46400.034.3731113
CACACGT46500.034.29919412
CGTCTGA47350.034.00848416
ACGTCTG47250.033.8476715
CACGTCT47200.033.83712814
CGTATGC46250.033.67598744
CACGATC47300.033.4400231
GATCTCG39700.032.69341739
TCCAGTC50300.031.57698225
CAGTCAC51250.031.24920327
CCAGTCA51450.030.91393926
AGCACAC52800.030.49832310
TCACGAT51850.030.46294430
TCGTATG50650.030.44668443
GAACTCC53100.030.03619621
GAGCACA53700.029.9052479
Produced by FastQC (version 0.11.2)