FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs16_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs16_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6983178
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC335470.48039732053228484TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG53700.039.4095711
ACACGTC53750.039.20919813
ACGTCTG54350.038.5734115
CACACGT54550.038.51319512
GATCGGA52250.038.2915081
CACGTCT55650.037.63279314
CGTCTGA56350.037.360516
GTCACAC55550.037.10678529
AGCACAC57800.036.6521410
CGTATGC51400.036.50763744
TCCAGTC58150.036.2042925
CAGTCAC57800.036.157127
GAACTCC59400.035.59055321
GAGCACA59650.035.589169
AGAGCAC60200.035.300818
CCAGTCA59250.035.12372626
AGTCACA59950.034.75030528
TCACACT59350.034.61950330
TCGGAAG62650.033.9683343
TCGTATG54200.033.6475243
Produced by FastQC (version 0.11.2)