FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs15_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs15_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431287
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC72700.13385409388235237TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13950.030.12050811
CTTAGGC13750.029.91865333
ACTTAGG14600.029.08088332
ACACGTC14600.028.93020413
ACGTCTG14600.028.92993715
CACGTCT14650.028.83120314
CACACGT14950.028.5472112
GATCGGA15900.025.7455941
CGTCTGA16200.025.66527716
GAACTCC16950.025.43838121
CGTATGC15450.025.2027544
CAGTCAC17300.024.79657227
CACTTAG17400.024.52763231
TCCAGTC17900.023.842525
AGCACAC18850.022.99100910
TTAGGCA17850.022.55360834
TCGGAAG19450.022.1758143
TAGGCAT17150.022.06314735
CCAGTCA19300.021.88502726
GAGCACA20250.021.5101439
Produced by FastQC (version 0.11.2)