FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs14_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs14_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8596274
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC539970.6281442401673096TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC91900.038.85053313
ACGTCTG91700.038.76733815
CACACGT92650.038.51229512
CACGTCT92200.038.41394814
GCACACG93150.038.35302711
CGTCTGA92500.038.33692616
CAGTCAC92800.037.81022327
CATCACG92500.037.7423733
CGATCTC70300.037.4256938
GATCTCG70800.037.40995839
TCCAGTC94000.037.39796425
CACGATC71000.037.11867536
CGTATGC92850.036.72347644
GTCACAT96150.036.58437729
AGTCACA96250.036.5235128
AGCACAC99450.035.94554510
CCAGTCA97600.035.8832926
TCGTATG94700.035.564743
TCACGAT86800.035.53221535
GTCTGAA99950.035.5234217
Produced by FastQC (version 0.11.2)