FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs13_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs13_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6552192
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT75890.11582383422219618TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG14800.032.2545533
GCACACG16450.029.95562711
ACGTCTG17000.028.59804515
CGTACGT16550.028.44518734
ACACGTC17200.028.39362513
CACACGT18050.027.05653212
GAACTCC18400.026.78098321
CACGTCT18550.026.44563314
CGTCTGA18850.025.7913416
AGCACAC19750.024.95038410
GTACGTA19100.024.76270735
TCCAGTC21400.023.4378325
GATCGGA20550.023.3506831
CAGTCAC21600.022.81342927
CTCGTAT18900.022.81325744
AGTCACC21550.022.76410528
GAGCACA22550.021.9498889
CCAGTCA22700.021.90175226
GTCTGAA22700.021.80467817
GTCACCG23350.021.10348329
Produced by FastQC (version 0.11.2)