FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs12_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs12_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7712422
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2337453.0307599869405486TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT119850.15539865427488278TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT118650.1538427228178126TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT105110.1362866295438709TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG346500.042.8158911
ACGTCTG345950.042.59118315
ACACGTC349000.042.5154913
CACACGT349650.042.43645512
CACGTCT348050.042.4290114
CGTCTGA346700.042.41655716
AGCACAC351850.042.21488610
AGTCACG343900.042.21179228
GTCACGT343750.042.0190329
TCACGTG344100.041.99547230
CACGTGA344500.041.953131
GAGCACA354500.041.9365439
CTCGTAT297450.041.83695644
TCCAGTC347100.041.816325
CGATCTC272950.041.80430640
AGAGCAC356650.041.7515838
GAACTCC349500.041.7434221
GATCGGA341650.041.6215061
GTCTGAA353350.041.60610217
CCAGTCA349150.041.57077426
Produced by FastQC (version 0.11.2)