FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs11_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs11_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682778
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT544870.8153345809182947TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC75850.041.8214333
ACGTCCG75950.041.65051332
GCACACG80350.041.01301211
CCGCACA73300.040.48528736
CACACGT81400.040.32151812
ACACGTC81850.040.20704313
GTCACGT80100.040.1242529
AGTCACG80450.040.0317228
AGCACAC83000.039.7565710
CACGTCC80700.039.55333731
ACGTCTG83150.039.44614815
CACGTCT83500.039.3071514
TCACGTC81700.039.14998230
CGTCTGA83750.039.0322216
CATCTCG68650.038.9659741
GATCGGA84150.038.950171
GAGCACA85150.038.830249
GAACTCC83300.038.66208621
GTCCGCA81350.038.29084434
TCCAGTC84600.038.17225
Produced by FastQC (version 0.11.2)