HJYMHAFXX_n01_vs10_170421.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJYMHAFXX_n01_vs10_170421.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10520316
Sequences flagged as poor quality	0
Sequence length	50
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT	513617	4.882144224565118	TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT	37871	0.35997968121870105	TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT	32104	0.3051619362003955	TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTAT	31683	0.3011601552652981	TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT	21233	0.20182853823022046	TruSeq Adapter, Index 16 (97% over 40bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	83635	43.045605	11
CGTCCCG	79725	42.973457	35
ACGTCTG	83255	42.92438	15
CACGTCT	83335	42.92277	14
CGTCTGA	82830	42.89232	16
GTCCCGA	74190	42.876125	36
ACACGTC	83950	42.78693	13
CCCGTCC	80355	42.770683	33
TCACCCG	80125	42.7452	30
CCGTCCC	80145	42.742764	34
CACACGT	84105	42.721153	12
ACCCGTC	79880	42.71933	32
AGCACAC	84335	42.672462	10
CACCCGT	80110	42.626884	31
CTCGTAT	68160	42.583923	44
CCCGATC	61020	42.554905	38
GAACTCC	82865	42.50765	21
TCCAGTC	82570	42.48921	25
GAGCACA	84870	42.46308	9
TCCCGAT	68760	42.445286	37