FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34791405
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT45050.014.1680611
CCCCCGG58350.012.1079721
GTCCCGT34950.011.7761051
GTCCCGA63900.011.1941151
CCCCCGA54050.011.1166561
CGCCTGT57250.010.9181761
ATCCCGA49050.010.4550021
ATCCCGT32350.010.2732981
ACCCGTT23400.010.2483432
CCCTCGT62800.09.9182261
CGGCGAC26650.09.73980915
CCCCTGT124000.09.7089631
CGGGCTA16250.09.6163931
ACGGCGA27250.09.606173514
TCCCGAA80450.09.5169162
CACGACG30000.09.23951622
CTCCCGT63600.09.1705631
ACCCCGT39250.09.0841151
CTCCCGA85050.09.00558951
CCCCCGC67050.08.9284761
Produced by FastQC (version 0.11.2)