FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36279668
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT45150.014.8185651
CCCCCGG56000.011.9081671
CGGCGAC29100.011.8679115
CCCCCGA45250.011.5271011
ACGGCGA29600.011.44454214
CACGACG33400.011.1314622
CTCCCGT57350.011.0905921
CGCCTGT57800.011.0423231
CGGGCTA14550.011.0420621
AACCCGT18300.010.9441141
ACCCGTT24550.010.9333782
GACGGCG34650.010.34796613
CCCCCGC56700.010.2473411
CCCCCTA30450.09.9742941
ATCCCGA45100.09.8086631
CGACGAC33650.09.80668624
CCCTCGT67350.09.8033381
GTCCCGA64600.09.6755611
ACCCCGT32550.09.5336331
CGCCCTA9750.09.4805811
Produced by FastQC (version 0.11.2)