FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33232570
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT34800.015.4945471
GACGGCG38950.012.820024513
ACGGCGA36600.012.7415814
CACGACG44500.011.91417822
CGGGCTA13400.011.8255051
CCCCCGA47300.011.4928521
CGGCGAC39750.011.45508915
CCCCCGC56800.011.1980281
CGCCCTA6500.011.1735881
ACCCGTT21300.010.6391562
CTCCCGT50650.010.4719991
ATCCCGT30900.010.4701011
CGCCTGT55700.010.4313561
CGACGAC44350.010.26808924
AACCCGT14900.010.1918931
GTCCCGT33500.010.1830751
CGTGTAT25250.010.1073123
ATTGCGC23300.010.00902236
GTCCCGA71500.09.8192121
TTAGCGG24050.09.78745659
Produced by FastQC (version 0.11.2)