FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37149659
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC53900.016.48808313
ACGTCTG55400.016.04148315
ATCGGAA54950.015.935042
CGTCTGA66150.013.60080316
CCCCCGT42800.013.4212871
AATCTCG54800.013.24920741
AACCCGT16700.012.6517951
CACGTCT72550.012.3405514
GATCGGA73800.011.958741
CACACGT77900.011.40837612
CGGGCTA15900.011.3504771
CCCCCGG78850.010.8299741
GTCCCGA73000.010.5823381
ACCCGTT24500.010.5064882
CCCCCGA55350.010.4179211
AGGGGGG119400.010.2671121
CGGAAGA96800.010.2502464
GTCCCGT42000.010.1135921
ACGGCGA31450.09.86225114
TCTCGTA69700.09.8182743
Produced by FastQC (version 0.11.2)