FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41435336
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1584000.38228240746014464TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC195500.039.4969544
TCTCGTA191000.039.24529341
TCGTATG190650.039.12692343
CTCGTAT191300.039.03650342
AATCTCG203100.038.96330339
ACACGTC260600.037.90057813
CGTCTGA259150.037.8914416
GATCGGA261550.037.7413561
ATCGGAA264750.037.5795252
ACGTCTG263850.037.31665415
ATCTCGT209400.037.06697540
TCGCGTA14600.036.4679541
TCGGAAG277450.035.866733
CACGTCT277200.035.662714
CGGAAGA280850.035.432524
CACACGT292250.033.84168212
GCACACG292950.033.75469611
CGCGTAT15650.033.60141442
ATCGCGT20250.031.83301740
AATCGCG25150.030.09162939
Produced by FastQC (version 0.11.2)