FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47382123
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1966650.415061604563392TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC238500.040.71695344
TCGTATG239200.040.0347243
CTCGTAT234700.040.00219742
TCTCGTA235100.039.83806641
ATCTCGT246750.038.30368840
GATCGGA274900.037.550991
CATCTCG252600.037.48553539
CGTCTGA274750.037.3012916
ATCGGAA282200.036.954882
ACACGTC278500.036.9178613
ACGTCTG281600.036.44880315
TCGGAAG294150.035.4677353
CGGAAGA299550.034.8577774
CACGTCT296650.034.61457414
GCACACG311250.033.28202411
CACACGT311450.033.19589212
CTTAGGC324800.031.26004633
ACTTAGG345200.029.6228932
TAGGCAT341800.029.53123535
TTAGGCA345000.029.45506734
Produced by FastQC (version 0.11.2)