FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33191686
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2601460.7837685618018921TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC303950.040.59255244
TCGTATG307850.039.96303643
CTCGTAT299150.039.74634642
TCTCGTA299350.039.4094841
ATCTCGT310850.037.8512340
ACGATCT312150.037.6230537
CGATCTC316600.037.1352638
GATCTCG318400.037.00082839
TCACGAT329700.036.88128735
CACGATC319700.036.7756236
ATCACGA334650.036.63844334
CATCACG360100.034.2755533
ACATCAC421050.029.63243732
GTCACAT423600.029.49360729
CGTCTGA439150.028.66669816
ATCGGAA449300.028.4599342
GATCGGA443150.028.4415531
TCACATC439700.028.4289830
ACACGTC450700.027.93723713
ACGTCTG453100.027.80369815
Produced by FastQC (version 0.11.2)