FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33153184
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA11650.012.83956417
GTATCCG30550.012.38669140
CGCGAAC13500.012.0598492
TATCCGT31950.011.706593541
GTCGTTT44600.011.6457071
CCCGTAT29050.011.5913511
CGCAAGT30800.011.5005632
TATAGCA56900.011.2892588
CCCGAAT70500.010.8949451
CGGCTAT25550.010.8534641
TTTCACG54800.010.83929224
CCCGTCT67700.010.8254091
CCGGCAT35650.010.7418351
CTCCGAT48500.010.5277361
GCCGATA15300.010.3569011
TACACCC46400.010.24129423
ATCGGCG12100.010.1811658
TTATAGC59700.010.1332777
TAAAGTA60600.09.98388235
GTTCGGC40250.09.89366733
Produced by FastQC (version 0.11.2)