FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34537988
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA20100.012.47822
GTCGTTT46850.012.1203431
CGCAAGT31600.011.76637652
GTACGTA12400.011.70816117
TCGAATG33800.011.5212542
CGTGTAA41000.011.37612933
TATAGCA62600.011.3507248
CCGCGTA8650.010.93629828
TTTCACG60700.010.8367424
TAATCCG16250.010.83065225
CGCATAT12000.010.6377941
CTCGAAT94800.010.4009851
CCCGAAT69600.010.1508071
GCCGATA13950.010.0974231
CCGATCG18150.010.05941919
AATTGGG66500.09.99348844
TTATAGC67950.09.8742727
TCGTTTT78250.09.8126032
GTATCAT38400.09.7973085
ATCGGCG13700.09.7950048
Produced by FastQC (version 0.11.2)