FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34488493
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT30050.013.0326741
CGCAAGT38600.012.9955342
CCCGAAT70600.012.7504391
GTACGTA9350.012.70429617
GTATCCG32600.012.41771640
TTTCACG59000.012.30497624
CCCGTAT26750.011.8480061
CGCGAAC12650.011.8267372
TAAAGTA64250.011.64222435
CCCGTTT57050.011.6122771
TATAGCA66300.011.5800548
TTCGACG37200.011.3542799
CTCCGGT87650.011.2745951
AATTGGG65200.011.1714844
CCGTTTA16250.011.1021492
CCCGTCT66000.010.9046151
GTCGTTT36800.010.7056181
CGGGCTT94750.010.6852621
GTTCGAC40600.010.6742888
CGATCTA12050.010.4069854
Produced by FastQC (version 0.11.2)