FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47721328
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG80700.013.6584432
CGAATGT76200.012.29943753
CTCGAAT161200.011.6046371
AATCCGA84200.011.49618924
CGCGAAA4550.011.1248481
CGCGAAC17550.010.5302622
CGGCTAT32600.010.3288381
CGATTAG43450.010.17746454
GACGTAT34400.010.16732117
AAATCCG93450.09.95807323
CCGTTTA25200.09.9527082
GTCGTTT86100.09.8920321
ATCCGAA99650.09.78005125
CTCCGAT86750.09.7671731
CGTGTAA46850.09.72084833
CGCTACT14950.09.715841
ACGCGAC11700.09.58953729
CTCGTTT103000.09.5509811
CCGGCAT44500.09.5449781
TATCATG100700.09.4604016
Produced by FastQC (version 0.11.2)