FastQCFastQC Report
Tue 4 Apr 2017
HJY7YBGX2_n01_ba65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7YBGX2_n01_ba65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47389148
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC28148335.9398261390983444TruSeq Adapter, Index 9 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350640.28501039942731193No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC666790.14070520955557167TruSeq Adapter, Index 9 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639820.13501403317063224No Hit
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG532820.11243502415363112TruSeq Adapter, Index 9 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3113700.043.11269844
ATCGGAA3387800.041.178922
GATCGGA3357300.041.1665231
TCGTATG3273300.040.9446843
TCGGAAG3388900.040.941583
CTCGTAT3175950.040.89067542
TCTCGTA3170150.040.8324641
CGGAAGA3391350.040.820754
GATCTCG3173050.040.6788539
ATCTCGT3174500.040.66016840
CGATCAG3322350.040.39374533
ACGATCA3341200.040.31186732
CACGATC3351750.040.18182431
GTCACGA3360750.040.08460629
TCACGAT3359550.040.0818830
AGTCACG3382950.039.82208328
GATCAGA3368650.039.7221834
AGATCTC3262100.039.58747538
CGTCTGA3449550.039.48774316
TCAGATC3272150.039.4807636