FastQCFastQC Report
Tue 20 Sep 2022
HJY7WDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJY7WDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005439746
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATGGGA1708118598.51742663127611No Hit
GTGGCAAT1657794618.266489249086618No Hit
AGCATCCG1643661878.196017224044786No Hit
GTCGATGC1513856797.5487523024289365No Hit
CAGCCACT1447838477.219556074361358No Hit
CCTCATTC1344250996.703023577154135ABI Solid3 Adapter A (100% over 8bp)
ACTAGGAG1297737756.471088211891857No Hit
TCTGTGAT1077890795.374835081183237No Hit
CACGGTGC1059369415.282479376969524No Hit
TGATTCTA945733514.715841061225282No Hit
CGCAGTTC928292694.628873501941653No Hit
ATATCCCG891797144.446890721991306No Hit
GAGCAAGA873847844.357387658955872No Hit
TTTCCACT832443954.15092974825283No Hit
AGATACAA823119984.104436354379504No Hit
GCGATGTG763469263.8069917658847476No Hit
GGGGGGGG470904172.3481342231261433No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)