FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9772433
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA879400.899878259590012TruSeq Adapter, Index 27 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132660.13574920390858652No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC119240.12201669737720382No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT114540.11720725023133952No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG112170.11478206092587179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG103000.062.10519848
CGTATGC102800.062.0895546
GTATGCC105500.060.63258447
ATGCCGT107750.059.43234349
TCGTATG107650.059.1307445
CTCGTAT108100.058.53172344
GCACACG123450.057.70291511
CACACGT124600.057.22600612
ACGTCTG124600.057.0887515
GCCGTCT114700.055.68332351
TATCTCG113850.055.36062241
CACGTCT130550.054.5388614
CCTTTAT118350.053.77896537
TCTCGTA118600.053.1461243
AGCACAC134400.053.12973410
ACACGTC134550.052.99413313
GTCACAT122650.052.93285429
TCCAGTC135100.052.3980625
CCAGTCA129850.051.51392426
CAGTCAC128050.051.2668827