Basic Statistics
Measure | Value |
---|---|
Filename | HJWYGBGX2_n01_jes_099.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9772433 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 87940 | 0.899878259590012 | TruSeq Adapter, Index 27 (97% over 44bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 13266 | 0.13574920390858652 | No Hit |
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC | 11924 | 0.12201669737720382 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT | 11454 | 0.11720725023133952 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG | 11217 | 0.11478206092587179 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 10300 | 0.0 | 62.105198 | 48 |
CGTATGC | 10280 | 0.0 | 62.08955 | 46 |
GTATGCC | 10550 | 0.0 | 60.632584 | 47 |
ATGCCGT | 10775 | 0.0 | 59.432343 | 49 |
TCGTATG | 10765 | 0.0 | 59.13074 | 45 |
CTCGTAT | 10810 | 0.0 | 58.531723 | 44 |
GCACACG | 12345 | 0.0 | 57.702915 | 11 |
CACACGT | 12460 | 0.0 | 57.226006 | 12 |
ACGTCTG | 12460 | 0.0 | 57.08875 | 15 |
GCCGTCT | 11470 | 0.0 | 55.683323 | 51 |
TATCTCG | 11385 | 0.0 | 55.360622 | 41 |
CACGTCT | 13055 | 0.0 | 54.53886 | 14 |
CCTTTAT | 11835 | 0.0 | 53.778965 | 37 |
TCTCGTA | 11860 | 0.0 | 53.14612 | 43 |
AGCACAC | 13440 | 0.0 | 53.129734 | 10 |
ACACGTC | 13455 | 0.0 | 52.994133 | 13 |
GTCACAT | 12265 | 0.0 | 52.932854 | 29 |
TCCAGTC | 13510 | 0.0 | 52.39806 | 25 |
CCAGTCA | 12985 | 0.0 | 51.513924 | 26 |
CAGTCAC | 12805 | 0.0 | 51.26688 | 27 |