FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223904
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA18205881.31074031727883TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCG14510.6480455909675575TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12960.5788194940688867TruSeq Adapter, Index 25 (97% over 43bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATA9200.4108903815921109TruSeq Adapter, Index 25 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8740.39034586251250536No Hit
GATCGGAAGAGCACACGTCTGAACTCTTCCGATCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA5610.25055380877518935TruSeq Adapter, Index 25 (100% over 40bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCT5140.2295626697155924TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG4160.18579391167643278TruSeq Adapter, Index 25 (97% over 43bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGC2600.11612119479777047TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT2470.11031513505788196TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2440.10897527511790767TruSeq Adapter, Index 25 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA150.002348233269.0357616
GCACGTC208.486849E-569.0203313
CACGCGT208.486849E-569.0203312
ACGTCAG150.002350315569.0203315
CGCACGT208.486849E-569.0203312
CGCGTCT208.486849E-569.0203314
GCGTCTG208.486849E-569.0203315
ACGCGTC208.486849E-569.0203313
CCCTAAT150.00235239969.0049251
GAACTAC150.00235239969.0049221
TGAACTA150.00235239969.0049220
CCTAATA150.00235239969.0049252
CTGCGTG150.00235239969.0049259
TCAGCTA150.00235239969.0049258
GCCCTCT150.00235239969.0049251
CAGCTAT150.00235239969.0049259
CTAATAC150.00235239969.0049253
CTGCTAG208.496246E-569.0049159
CCCTCTT253.0849787E-669.0049152
GAACGGA208.496246E-569.004911
Produced by FastQC (version 0.11.2)