FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9109426
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA756250.8301840313538965TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145960.16022963466633355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91700.059.5607748
CGTATGC91950.059.35902846
GTATGCC95750.057.1480247
ATGCCGT95950.056.92196349
TCGTATG99200.054.9857145
TATCTCG99550.054.54769541
AGTCACG102550.054.1925328
CACACGT116300.053.8355712
ACGTCTG115950.053.7918915
ACGAGTG102400.053.5322432
GCACACG117900.053.39756811
CGAGTGG102700.053.24179533
TGCCGTC103400.053.02208750
CTCGTAT102350.052.9220544
GTCACGA106050.052.2419329
CACGAGT106050.051.75427231
GCCGTCT105900.051.73865551
ACACGTC121650.051.58195513
CACGTCT123050.050.7713614
TCTCGTA109600.049.5154443
Produced by FastQC (version 0.11.2)