FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8930046
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1470981.6472255574047436TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132370.14822991953232942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA171100.064.5187535
CGTATGC170300.064.5071146
CGTACGT172550.063.97621534
TATGCCG171950.063.88990848
CCGTACG174600.063.32420733
TACGTAA174400.063.1990236
GTATGCC174750.062.8848947
TCGTATG176350.062.4299945
ACGTAAT177000.062.09525737
ATGCCGT178000.061.77617349
GCACACG197200.061.4532811
CTCGTAT175700.061.42210844
ACGTCTG199300.060.67033815
CACACGT199850.060.6038912
ACCGTAC182350.060.5382932
GCCGTCT185550.059.31962651
ACACGTC206600.058.5747413
TCTCGTA184400.058.44904343
CACGTCT207000.058.4458714
CGTAATC184750.058.24070738
Produced by FastQC (version 0.11.2)