FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10742876
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1163161.0827268228731302TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170280.15850504092200263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC140650.062.89491746
TATGCCG144600.061.2742748
GTATGCC146100.060.61985847
TCGTATG147550.060.0700745
ACGTCTG175950.059.48742315
GCACACG177550.059.1809311
CACACGT177850.059.04231312
ATGCCGT150200.059.0345849
CTCGTAT147650.058.04239344
GTCACGT155600.057.97526629
CGTTTCG154950.057.64042733
AGTCACG156800.057.59758428
ACACGTC182650.057.32151413
CACGTCT184400.056.74168414
CACGTTT162200.055.50988431
GCCGTCT160050.055.29747851
AGCACAC192250.054.61964410
TGCCGTC162050.054.5716650
TCTCGTA157750.054.36969443
GTTTCGG166450.053.82311634
Produced by FastQC (version 0.11.2)