FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11349917
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1166001.0273202878928542TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171780.1513491244032886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG139750.061.3519848
CGTATGC140050.061.31720746
GTATGCC146150.058.82980747
ACGTCTG172100.058.5316715
TCGTATG147700.058.2347745
ATGCCGT148450.057.89560349
GGCCTTA148000.057.8303636
AGTCACG150950.057.5907228
GCACACG175900.057.50102611
CTCGTAT149800.056.70340744
TCACGTG152800.056.44291330
CGTGGCC152500.056.35035333
GTCACGT153400.056.31160429
CACACGT180400.056.08580812
GCCTTAT152600.055.92960437
ACACGTC182450.055.4936913
CACGTGG156550.055.15698231
TATCTCG152900.055.14590541
ACGTGGC156500.055.08643332
CACGTCT183550.054.91776314
Produced by FastQC (version 0.11.2)