FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11377877
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA636640.5595419954003722TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169620.14907877805323436No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG132540.11648921850710814No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT116130.10206649272091797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77800.059.16057248
CGTATGC78750.058.4450946
GTATGCC81950.056.4157747
TCGTATG83250.055.4921445
GCACACG106750.054.8060111
ACGTCTG106950.054.5453715
ATGCCGT84400.054.5347549
CACACGT109500.053.4615812
GTCACGT87550.053.27216329
AGTCACG88550.053.21502728
CACGTGA88050.053.00883531
CTCGTAT84950.052.6742544
TCACGTG89100.052.38414830
CACGTCT114500.050.9777214
GCCGTCT90700.050.55746551
AGCACAC116950.050.2914810
ACGTGAA96500.048.51075732
ACACGTC122000.047.89955513
TCCAGTC124600.046.3467325
ACTCCAG125200.046.2903523
Produced by FastQC (version 0.11.2)