FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12516077
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA747790.5974635662596196TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168700.1347866428114816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC91250.060.526733
TATGCCG94750.058.1540848
CCGCACA94600.058.09157636
CGTATGC95400.057.86450246
TCCGCAC95800.057.5437435
GTCCGCA96550.057.20392634
CGCACAT96000.057.02948437
GTATGCC97900.056.35206247
ACGTCCG101400.054.77403332
ATGCCGT101700.054.2136449
GCACACG125200.054.19671611
CACACGT126350.053.62195612
ACGTCTG126550.053.48418815
TCGTATG104150.052.9357945
AGTCACG107300.052.40419428
ACACGTC131150.051.73874713
GTCACGT108250.051.62582429
CACGTCT136200.049.8459214
CTCGTAT109550.049.72708544
TCACGTC113900.048.97426230
Produced by FastQC (version 0.11.2)