FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9279747
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG584670.6300495045823986TruSeq Adapter, Index 16 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113420.12222315974778192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC71550.059.4961633
CGTATGC72500.058.9589144
TATGCCG73100.058.24183746
GTATGCC75700.056.05793845
ATGCCGT77550.055.47844347
ACGTCTG97600.054.50387215
GCACACG98200.054.27390311
TCGTATG78950.054.14241843
CACACGT99400.053.61868312
CTCGTAT79200.052.83837542
CACGTCT104900.050.7761714
GCCGTCT85850.049.71368849
CGTCCAT86550.048.7873435
ACACGTC109550.048.7140613
CCGTCCA87800.048.56346534
AGCACAC111300.048.1955510
CATCTCG86750.048.04014239
TCCAGTC110550.047.8061125
TCTCGTA89100.046.89050741
AGTCACC92950.046.6137828
Produced by FastQC (version 0.11.2)