FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8976352
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA692450.7714158268303204TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128340.14297567653318408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC85400.060.19599546
TATGCCG85200.060.17848
GTATGCC89150.057.665247
ATGCCGT91150.056.36269449
GCACACG120950.055.9583111
TCGTATG92600.055.1432545
ACGTCTG122300.055.03433615
CACACGT124100.054.42735312
ACACGTC128350.052.67887513
TGCCGTC97850.052.43377350
CTCGTAT94900.052.31561344
CACGTCT130050.052.0715814
GCCGTCT100150.051.19572451
AGCACAC133750.050.83488510
GTCACAT108100.048.92098229
TCTCGTA102250.048.68918643
TCCAGTC134150.048.47375525
CGTCTGA139300.048.39419616
CTGAACT139700.048.18181219
GAGCACA143750.047.4665269
Produced by FastQC (version 0.11.2)