FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7573594
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA464430.6132227315063364TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107380.14178209183117024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58350.057.76731546
TATGCCG59900.056.33270348
GTATGCC61000.055.37196447
ACGTCTG84800.055.12359215
GCACACG85550.055.0410411
CACACGT85700.054.62304312
ACACGTC86450.054.0697113
ATGCCGT64100.052.74963849
TCCGTAT63300.052.3737637
TCGTATG64050.052.19619845
CACGTCT89900.052.0337914
CGTATCT64800.051.0023639
TTCCGTA66200.050.59988836
CCGTATC65550.050.3655238
CTCGTAT66150.050.27773344
TATCTCG66700.049.44771241
AGCACAC95700.049.2390810
GTTCCGT69050.049.01133735
GCCGTCT69700.048.56357251
CGTCTGA97250.048.0679116
Produced by FastQC (version 0.11.2)