FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115613
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA817781.1492755437936268TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129750.18234549855367344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG94850.062.05809448
CGTATGC95250.061.75735546
GTATGCC97550.060.3732747
TCGTATG98850.059.50864445
ATGCCGT100200.058.98689349
GCACACG121900.057.5022911
ACGTCTG122700.057.3286115
CACACGT122950.056.98316612
CTCGTAT101350.056.78080744
ACACGTC124550.056.30772813
GCCGTCT107550.055.0523451
CACGTCT129050.054.5604114
TGCCGTC108450.054.50041250
TCTCGTA107500.053.49994343
AGCACAC133000.052.83254210
CGTCTGA133650.052.71094516
CACAGTC115200.052.1043731
CTGAACT135600.052.05616419
TCCAGTC136000.051.5185525
ACTCCAG137200.051.2942523
Produced by FastQC (version 0.11.2)