FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8909992
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG707490.7940411169841679TruSeq Adapter, Index 12 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143820.1614142863427936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC84750.059.76049444
TATGCCG84750.059.6807546
GTATGCC88200.057.61915645
ATGCCGT88550.057.1595647
TCGTATG90150.056.14224643
CTCGTAT90950.054.62176542
GCACACG98550.053.6961311
ACGTCTG98300.053.37917715
CACACGT99450.052.9326912
TGCCGTC99150.051.2937348
ACACGTC102800.051.2757213
GTCACCT100550.050.60477429
GCCGTCT100400.050.48328849
CACGTCT105150.049.96656414
TCTCGTA101450.049.10502641
AGTCACC106850.047.91135828
AGCACAC112250.047.173310
CAGTCAC109500.046.62584727
CGTCTGA113650.046.3519716
TCCAGTC113800.046.1076925
Produced by FastQC (version 0.11.2)