FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764565
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG477930.5452980267702962TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113350.12932758214469287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63550.057.81870344
TATGCCG63500.057.64723246
GCACACG89650.055.06448711
ATGCCGT68100.053.90620447
ACGTCTG91700.053.68424615
CACACGT92200.053.57927712
TCGTATG68700.053.38305743
GTATGCC69150.053.3362345
ACACGTC96150.051.34227813
AGTCACG75850.050.3931428
CTCGTAT72450.049.95128342
CACGTCT100750.048.9986714
CACGGCT75650.048.83911531
AGCACAC101850.048.36675610
CGGCTAC76800.048.15326733
GGCTACA76850.047.71792634
GTCACGG79300.047.67844829
ACGGCTA77950.047.3095532
GCTACAT77200.047.05499335
TGCCGTC78250.046.9582648
Produced by FastQC (version 0.11.2)