FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9546359
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG899770.942526883809838TruSeq Adapter, Index 10 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161590.1692687232902094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG108150.060.35580446
CGTATGC108150.060.19472544
TCGTATG111700.058.46726643
ATGCCGT111900.058.24096347
GTATGCC113450.057.8396845
CTCGTAT114050.056.23317742
ACGTCTG131300.054.83577315
GCACACG132650.054.3537311
CACTAGC121100.054.32464231
TATCTCG118300.054.1537939
CACACGT133300.054.1145712
TAGCTTA121000.053.99918434
GCCGTCT120550.053.94828849
TCACTAG125450.052.77063430
ACACGTC138900.051.9579513
TGCCGTC126050.051.81303448
CACGTCT139250.051.803414
TCTCGTA124200.051.6370941
AGCACAC143400.050.23045310
AGTCACT134500.049.47612828
Produced by FastQC (version 0.11.2)