FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11619285
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1156270.9951300790022793TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG139750.061.89352846
CGTATGC141350.061.46010644
GTATGCC143250.060.52554745
ATGCCGT145250.059.55065547
TCGTATG149150.058.29172543
AGTCACG154500.056.892128
CTCGTAT150750.056.43542
ACGTCTG174400.055.90030315
GTCACGA157950.055.4089629
GCCGTCT157250.055.16149549
GCACACG177250.055.13606311
CACACGT180100.054.3404112
TGCCGTC161650.053.68184748
TCTCGTA158300.053.6770741
CACGTCT184550.052.88171814
ACACGTC186450.052.39743413
AGCACAC191750.051.16460410
TCCAGTC192100.050.3719125
CGATCAG173500.049.98668333
ACTCCAG194950.049.9369823
Produced by FastQC (version 0.11.2)