FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361716
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG776140.7490458144191561TruSeq Adapter, Index 8 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141420.1364831848315472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC95350.059.2685744
TATGCCG95450.059.13561246
GTATGCC100050.056.45121845
ATGCCGT100900.055.8741647
TCGTATG102000.055.3365743
ACGTCTG118100.053.7189415
GCACACG119650.052.93322811
CTCGTAT105050.052.01963442
CACACGT121950.051.9917212
GCCGTCT110950.050.65971449
ACACGTC125200.050.61576513
TGCCGTC111400.050.60896348
CACGTCT126650.050.03699514
TCTCGTA113300.048.32244541
GTCACAC118250.048.25013429
TCCAGTC135250.046.701425
AGCACAC136050.046.57712610
CGTCTGA138600.045.94861616
CAGTCAC126500.045.7037527
CCAGTCA130900.045.38038326
Produced by FastQC (version 0.11.2)