FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11529115
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG668480.5798190060555385TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169880.14734869068441073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG83650.057.90836346
CGTATGC84400.057.4735444
GCACACG121300.054.48995211
ATGCCGT89050.054.08801747
ACGTCTG121950.054.05902515
CACACGT122500.054.0406612
GTATGCC90100.053.8770545
TCGTATG93200.052.11976243
ACACGTC129550.051.04677613
CACGTCT132500.049.80655314
CTCGTAT96400.049.7092442
AGCACAC138150.048.06798610
TGCCGTC102300.047.15075348
GCCGTCT102750.046.9770249
CGTCTGA142900.046.2563516
TCTCGTA105900.045.15144341
TCCAGTC143600.044.8036825
CATCTCG107150.044.55993339
CTGAACT149000.044.339119
GAGCACA152200.043.789369
Produced by FastQC (version 0.11.2)